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Determination of clenbuterol in human urine and serum by solid-phase microextraction coupled to liquid chromatography. Clenbuterol LC-MS/MS Stop-and-go extraction (StAGE) Urine microsampling Volumetric absorptive microsampling (VAMS) 1. Introduction Clenbuterol (1- (4-amino-3,5-dichlorophenyl)-2- (tert -butylamino)ethanol, CBT, Fig. 1) is a sympathomimetic agent with strong selectivity toward β2 -adrenergic receptors [ 1]. A method for clenbuterol residue analysis in urine and animal tissues has been developed. The detection limits are 0. 25 micrograms/l and 0. The recovery in urine varies from 85% to 90% and in animal tissues from 70% to 74%. Clenbuterol ( Figure 1) is an orally bioavailable, directly acting sympathomimetic agent with a mainly β-adrenergic activity that binds to β2 receptors with high affinity and selectivity [1]. A urine analysis collected from 47 of the patients showed that all had traces of clenbuterol, ranging from 11 to 486 parts per billion. Clenbuterol is one of the most misused anabolic agents in professional sports. Therefore, the monitoring of clenbuterol in body fluids such as human urine is related to the development of Ambroxol with Clenbuterol Pediatric is typically prescribed by a doctor and should only be used under the supervision of a healthcare professional, clenbuterol urine analysis.
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